Cobbetts is to axe up to 40 fee-earners in response to the worsening economic climate.
The news comes just weeks after the firm announced that six lawyers were being made redundant in the domestic conveyancing and professional support departments.
Managing partner Michael Shaw said the job losses were the result of firm-wide review of services.
The exact number of redundancies and the practice groups affected will be decided after a consultation with staff.
Shaw said: “While this has been a very difficult decision for the firm, it is an essential move in response to our changing economy.
“Flexibility is key for those firms intending to preserve their business in the coming months, until we begin to see economic recovery.”
The firm confirmed that the job losses would be in Birmingham, Leeds and Manchester.
More than five per cent of the 740 jobs at Cobbetts are under threat as a result of the consultation.
More than 440 law firm employees have been axed since the start of the credit crunch last year.